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Benjamin Aroeti Lab

Department of Cell and Developmental Biology/Biological Chemistry

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Benjamin Aroeti Lab

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HOME / LAB PROTOCOLS

LAB PROTOCOLS

AROETI_Caco-2 BBe cell culturing  - EZR

AROETI_Collagen coating of coverslips plates and Transwells - EZR

AROETI_Counting of cells using Hemocytometer - IN

AROETI_Freezing of HeLa cells - IN

AROETI_Invasion assay - RR

AROETI_Media preparation for HeLa cells - IN

AROETI_PEI Linear transfection of HeLa cells - IN

AROETI_Western blotting - IN

AROETI_Electroporation of EPEC with plasmid cDNA

AROETI_Invasion assay MDCK cells- IZ

AROETI_MDCK Cell Culturing  - EZR

AROETI_Effector expression levels in EPEC complemented baacteria - EZR IN

AROETI_Preparation of competent bacteria- RS EZR

AROETI _TCA Precipitation (Mrio Lebendiker)

AROETI_EspH Effector translocation assay - RR

Gentamycin protection assay Invasion assay

AROETI_Bacterial Activation - SB

AROETI _Lentiviral Knockout of gene of interest by gRNA - IN

AROETI _Lentiviral Knockdown of gene of interest by shRNA - IN

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